Gastrointestinal parasites among Musahar Community in Balan-Bihul, Saptari, Nepal
Abstract
Gastrointestinal parasites inhabiting the intestinal tract can directly or indirectly affect the growth, well-being, and survival of hosts, including humans. In this context, the Musahar, highly marginalized ethnic tribes with existing discrepancies, like illiteracy, low socio-economic conditions, and lack of awareness concerning healthy hygienic practices, may be at an increased risk of infectious parasitic diseases. Therefore, the current aim is to determine the diversity and frequency of gastrointestinal parasites among the Musahar residing in the Saptari district in eastern Nepal. Using purposive sampling and non-invasive techniques 200 fresh fecal samples and epidemiological data for microscopic examination and identification of risk factors were collected. The coproscopy was carried out using direct wet mount and concentration techniques. The results showed an 81% (162/200) prevalence and the presence of nine different species of parasites, including Ancylostoma sp. (41.5%), Ascaris lumbricoides (29%), Entamoeba sp. (31.5%), Entamoeba coli (21.5%), Trichuris trichuria (16%), Strongyloides stercoralis (8.5%), Giardia sp. (7%), Hymenolepis nana (14%), and Blastocystis sp. (2%). The prevalence of helminths (67%) was higher than that of single-celled protozoa (42%). Furthermore, parasitism was significantly higher among individuals who consumed game meat, defecated in open spaces, lived in mud homes, and had an unknown history of medication. In conclusion, the Musahar community exhibits a higher prevalence of gastrointestinal parasites. Therefore, implementing community-based health awareness programs concerning the cause, transmission route, effects as well as preventive measures for parasitism is highly recommended. In addition, the One Health study approach is essential to address the root causes of parasite transmission and ways to prevent them.
References
Adhikari R.B., Adhikari Dhakal M. and Ghimire T.R. 2024. Intestinal parasitism in working horses and associated zoonotic risks in lowlands of Nepal. Problems of Infectious and Parasitic Diseases, 52(1):34–46. https://doi.org/10.58395/pkz5qg48
Adhikari R.B., Adhikari Dhakal M. and Ghimire T.R. 2023. Prevalence of intestinal parasites in street dogs (Canis lupus familiaris) with highlights on zoonosis in Lalitpur, Nepal. Veterinary Medicine and Science, vms3:1258. https://doi.org/10.1002/vms3.1258
Adhikari R.B. and Ghimire, T. R. 2021. A case study of multiple parasitisms in a calf buffalo (Bubalus bubalis). Agricultural Science Digest, 41:237–241. https://doi.org/10.18805/ag.D-5172
Adhikari R.B., Maharjan M. and Ghimire T.R. 2020. Prevalence of gastrointestinal parasites in the frugivorous and the insectivorous bats in southcentral Nepal. Journal of Parasitology Research, 2020:1–12. https://doi.org/10.1155/2020/8880033
Adhikari R.B., Parajuli R.P., Maharjan M. and Ghimire T.R. 2021. Prevalence and risk factors of gastrointestinal parasites in the Chepangs in Nepal. Annals of Parasitology, 67(3):387–405. https://doi.org/10.17420/ap6703.353
Amadi E.C. and Uttah E.C. 2010. Impact of physico-chemical factors of contaminated foci on the survival of geohelminths in Abua Communities, Niger Delta Nigeria. Journal of Applied Sciences and Environmental Management, 14(4).
Amer O.H., Ashankyty, I.M. and Haouas N.A.S. 2016. Prevalence of intestinal parasite infections among patients in local public hospitals of Hail, Northwestern Saudi Arabia. Asian Pacific Journal of Tropical Medicine, 9(1):44–48.
Aryal M., Adhikari R.B., Kandel P., Ghimire T.R., Khadka D., Maharjan J., et al. 2022. First report on the molecular detection of Entamoeba bovis from the endangered wild water buffalo (Bubalus arnee) in Nepal. Veterinary Medicine and Science, 8(2):799–807. https://doi.org/10.1002/vms3.697
Baral R., Jha P., Amatya R. and Khanal B. 2017. Prevalence of intestinal parasitic infections among patients attending in a tertiary care hospital of eastern region of Nepal–A retrospective, laboratory based study. Asian Journal of Medical Sciences, 8(3):55–59.
Boutahar M., Belaouni M., Ibrahimi A., Eljaoudi R., Aanniz T. and Er-Rami M. 2023. Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones’ culture medium. Parasite, 30:64. https://doi.org/10.1051/parasite/2023065
Chaudhary S. and Subedi J.R. 2020. Comparative prevalence of intestinal helminths in Satar and Chaudhary communities of Birtamode municipality, Jhapa, Nepal. National Journal of Health Sciences, 5(1):4–12.
Chopra P., Shekhar S., Dagar V.K. and Pandey S. 2023. Prevalence and risk factors of soil-transmitted helminthic infections in the pediatric population in India: A systematic review and meta-analysis. Journal of Laboratory Physicians, 15(01):004–019. https://doi.org/10.1055/s-0042-1751319
Coello Peralta R.D., Salazar Mazamba M.D.L., Pazmiño Gómez B.J., Cushicóndor Collaguazo D.M., Gómez Landires E.A. and Ramallo G. 2023. Hymenolepiasis caused by Hymenolepis nana in humans and natural infection in rodents in a marginal urban sector of Guayaquil, Ecuador. American Journal of Case Reports 24:939476. https://doi.org/10.12659/AJCR.939476
DeVeale B., Brummel T. and Seroude L. 2004. Immunity and aging: The enemy within? Aging Cell, 3(4):195–208.
Dhakal N. and Subedi J.R. 2019. Intestinal parasites in Meche community in relation to their socio-economic status. Janaki Medical College Journal of Medical Science, 7(1):14–24.
Dhanabal J., Selvadoss P.P. and Muthuswamy K. 2014. Comparative study of the prevalence of intestinal parasites in low socioeconomic areas from South Chennai, India. Journal of Parasitology Research 2014.
Ezeamama A.E., McGarvey S.T., Acosta L.P., Zierler S., Manalo D.L., Wu H.-W., et al. 2008. The synergistic effect of concomitant schistosomiasis, hookworm, and Trichuris infections on children’s anemia burden. PLoS Neglected Tropical Diseases 2(6):e245. https://doi.org/10.1371/journal.pntd.0000245
Gupta R., Rayamajhee B., Sherchan S.P., Rai G., Mukhiya R.K., Khanal B. and Rai S.K. 2020. Prevalence of intestinal parasitosis and associated risk factors among school children of Saptari district, Nepal: A cross-sectional study. Tropical Medicine and Health, 48(1), 73. https://doi.org/10.1186/s41182-020-00261-4
Gurung Y. 2014. Social demography of Nepal: Evidence from population and housing census 2011. Population Monograph of Nepal 2.
Hamory J., Miguel E., Walker M., Kremer M. and Baird S. 2021. Twenty-year economic impacts of deworming. Proceedings of the National Academy of Sciences, 118(14):e2023185118. https://doi.org/10.1073/pnas.2023185118
Haque R. 2007. Human intestinal parasites. Journal of Health, Population, and Nutrition 25(4):387–391.
Hoarau A.G., Mavingui P. and Lebarbenchon C. 2020. Coinfections in wildlife: Focus on a neglected aspect of infectious disease epidemiology. PLOS Pathogens, 16(9):e1008790. https://doi.org/10.1371/journal.ppat.1008790
Issa R. 2019. Musca domestica acts as transport vector hosts. Bulletin of the National Research Centre, 43(1):73. https://doi.org/10.1186/s42269-019-0111-0
Jourdan P.M., Lamberton P.H., Fenwick A. and Addiss D.G. 2018. Soil-transmitted helminth infections. The Lancet, 391(10117):252–265.
Kang G., Mathew M.S., Prasanna Rajan D., Daniel J.D., Mathan M.M., Mathan V. I. and Muliyil J. P. 1998. Prevalence of intestinal parasites in rural Southern Indians. Tropical Medicine & International Health, 3(1):70–75.
Karki M. 2019. Balan-Bihul rural municipality profile | facts & statistics. https://www.nepalarchives.com/content/balan-bihul-rural-municipality-saptari-profile/
Kattula D., Sarkar R., Rao Ajjampur S.S., Minz S., Levecke B., Muliyil J. and Kang G. 2014. Prevalence and risk factors for soil transmitted helminth infection among school children in south India. The Indian Journal of Medical Research, 139(1):76–82.
Khadka S., Sapkota S., Adhikari S., Dubey A. K., Thapa A., Bashyal R. and Bhusal H. 2021. Intestinal parasitoses among Chepang and Musahar community people of Makwanpur and Nawalparasi Districts of Nepal. Acta Parasitologica, 66:146–154.
Lanker K.C., Muhummed A.M., Cissé G., Zinsstag J., Hattendorf J., Yusuf R.B., Hassen S.B., Tschopp R. and Vonaesch P. 2023. Prevalence and associated risk factors of intestinal parasitic infections among children in pastoralist and agro-pastoralist communities in the Adadle woreda of the Somali Regional State of Ethiopia. PLOS Neglected Tropical Diseases, 17(7):e0011448. https://doi.org/10.1371/journal.pntd.0011448
Li L.H., Zhang X.P., Lv S., Zhang L., Yoshikawa H., Wu, Z., et al. 2007. Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China. Parasitology Research, 102(1):83–90. https://doi.org/10.1007/s00436-007-0727-0
Morand S., Jittapalapong S. and Kosoy M. 2015. Rodents as hosts of infectious diseases: Biological and ecological characteristics. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 15(1):1–2. https://doi.org/10.1089/vbz.2015.15.1.intro
Nagel R., Cuttell L., Stensvold C.R., Mills P.C., Bielefeldt‐Ohmann H. and Traub R.J. 2012. Blastocystis subtypes in symptomatic and asymptomatic family members and pets and response to therapy. Internal Medicine Journal, 42(11):1187–1195. https://doi.org/10.1111/j.1445-5994.2011.02626.x
Ngui R., Ishak S., Chuen C.S., Mahmud R. and Lim Y.A. 2011. Prevalence and risk factors of intestinal parasitism in rural and remote West Malaysia. PLoS Neglected Tropical Diseases, 5(3):e974.
Njunda A.L., Fon S.G., Assob J.C.N., Nsagha D.S., Kwenti T.D.B. and Kwenti T.E. 2015. Coinfection with malaria and intestinal parasites, and its association with anaemia in children in Cameroon. Infectious Diseases of Poverty, 4:1–7.
NPHC. 2021. National Population and Housing Census 2021. National Report on caste /ethnicity, language, and Religion (pp. 1–502) [Government of Nepal]. https://censusnepal.cbs.gov.np/results/files/result folder/Caste%20Ethnicity_report_NPHC_2021.pdf
Opara K. 2012. The impact of intestinal parasitic infections on the nutritional status of rural and urban school-aged children in Nigeria. International Journal of MCH and AIDS (IJMA), 1(1). https://doi.org/10.21106/ijma.8
Parajuli R.P., Umezaki M. and Watanabe C. 2009. Behavioral and nutritional factors and geohelminth infection among two ethnic groups in the Terai region, Nepal. American Journal of Human Biology: The Official Journal of the Human Biology Association 21(1):98–104.
Path R. and Kathmandu N. 2014. Population monograph of Nepal. Central Bureau of Statistics. 2:165–170.
Pestehchian N. 2015. Prevalence of intestinal parasitic infection among inhabitants and tribes of Chelgerd, Iran 2008-2009. Journal of Clinical and Diagnostic Research. https://doi.org/10.7860/JCDR/2015/11580.5879
Poudel B. and Kattel S. 2019. Social changes in Musahar community: A case study of Dhanusa District of Nepal. Nepal Journal of Multidisciplinary Research 2(4):9–16.
Rai S.K., Hirai K., Abe A., Nakanish M., Rai G., Uga S. and Shrestha H.G. 2004. Study on enteric parasitosis and nutritional status of school children in remote hilly areas in Nepal. Nepal Medical College Journal: NMCJ, 6(1):1-6.
Rana M.K. 2017. Occupation and Educational Status of Musahar. Central Department of Rural Development Tribhuvan University, Kritipur
Sahay G.R. 2019. Substantially present but invisible, excluded and marginalised: A study of Musahars in Bihar. Sociological Bulletin, 68(1) 25–43.
Shahnazi M., Mehrizi M.Z., Alizadeh S.A., Heydarian P., Saraei M., Alipour M. and Hajialilo E. 2019. Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker. African Health Sciences, 19(1):1346–1352.
Steinbaum L., Njenga S.M., Kihara J., Boehm A.B., Davis J., Null C. and Pickering A.J. 2016. Soil-transmitted helminth eggs are present in soil at multiple locations within households in rural Kenya. Plos One, 11(6):e0157780. https://doi.org/10.1371/journal.pone.0157780
Tetra Tech International Development, NIDR, and JWASH. 2022. Ethnographic Participatory Research for Musahar Girl’s Education (PEAR-MGE) (pp. 1–68). https://girlseducationchallenge.org/media/qweojyb5/rrlf-nidr-report-may-2023.pdf
Thapa N., Subedi J. R. and Chhetri B. 2021. Prevalence of intestinal parasites among Sarki ethnic group of Pala rural municipality, Baglung, Nepal.
Thompson R.C.A. 2015. Neglected zoonotic helminths: Hymenolepis nana, Echinococcus canadensis and Ancylostoma ceylanicum. Clinical Microbiology and Infection 21(5):426–432. https://doi.org/10.1016/j.cmi.2015.01.004
Tigabu A., Taye S., Aynalem M. and Adane K. 2019. Prevalence and associated factors of intestinal parasitic infections among patients attending Shahura Health Center, Northwest Ethiopia. BMC Research Notes, 12(1):333. https://doi.org/10.1186/s13104-019-4377-y
Torgerson P.R., Devleesschauwer B., Praet N., Speybroeck N., Willingham A.L., Kasuga F., et al. 2015. World Health Organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases 2010: A data synthesis. PLoS Medicine, 12(12):e1001920. https://doi.org/10.1371/journal.pmed.1001920
Wawrzyniak I., Poirier P., Viscogliosi E., Dionigia M., Texier C., Delbac F. and Alaoui H.E. 2013. Blastocystis, an unrecognized parasite: An overview of pathogenesis and diagnosis. Therapeutic Advances in Infectious Disease, 1(5):167–178. https://doi.org/10.1177/2049936113504754
Welch V.A., Ghogomu E., Hossain A., Awasthi S., Bhutta Z.A., Cumberbatch C., et al. 2017. Mass deworming to improve developmental health and wellbeing of children in low-income and middle-income countries: A systematic review and network meta-analysis. The Lancet Global Health, 5(1):e40–e50. https://doi.org/10.1016/S2214-109X(16)30242-X
WHO. 2023. Soil-transmitted helminth infections. https://www.who.int/news-room/fact-sheets/detail/soil-transmitted-helminth-infections
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